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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۵-۱۰۵
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عنوان انگلیسی Ps-73: The Study of Bone Marrow Stromal Cells Cultured on PLGA Nanofibers
چکیده انگلیسی مقاله Objective: The most important factor in choosing a polymer for the preparation of nano-fiber for use in medical and biological purposes, having the properties of biocompatibility and biodegradation. PLGA for the above benefits and much attention in tissue engineering, the present study aimed at examining bone marrow stromal cells cultured on PLGA nanofibers have been consideration. Materials and Methods: In this study, the PLGA nanofiber by electro spinning technique with solvent hexa fluoro propanol was prepared. For study Properties of the polymer using scanning electron microscopy (SEM) and were analyzed invert microscope. After harvesting the BMSCs rat cells to achieve passage of two, cells in two groups PLGA nanofibers and without nanofibers were cultured. Cell proliferation on day’s two, four and six by Acridine Orange and morphology of cells was examined by SEM. Results: The results showed significant reduction in cell proliferation in PLGA nanofibers alone (p< 0.05). Conclusion: According to the survey results of BMSc cell culture, we can conclude that PLGA nanofibers can be used as biodegradable and biocompatible scaffold with bone marrow cells used in tissue engineering.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/399
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