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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۷-۱۰۷
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عنوان انگلیسی Ps-75: ProMyelocytic Leukemia (PML) Expression during Human Embryonic Stem Cells (hESCs) Neural Differentiation Indicates A Role for PML Nuclear Bodies (PML-NBs) in Cellular Pluripotency Than Neural Differentiation
چکیده انگلیسی مقاله Objective: Pro-myelotic leukemia (PML) is one of the major proteins in promyelotic leukemia nuclear bodies (PML-NBs). Retinoic acid (RA) exerts its tumor growth suppressor activity and terminal myeloid differentiation of granulocyte/monocyte progenitor (GMP) cells via PML-NBs in RA pathway. In addition, RA as a natural morphogen guides posterior patterning in embryo neural development. Based on these two scenarios the aim of this study was to define if there was any revenue for PML-NBs in RA dependent neural development. Materials and Methods: For this reason, RA was used as a neural inducer for in vitro neural differentiation of human embryonic stem cells (hESCs). PML mRNA and protein levels were assessed by quantitative real time RT-PCR (q-RT-PCR) and western blotting in hESCs, neural precursor cells (NPCs) and mature neural cells (MNCs) obtained from this differentiation process. Results: qRT-PCR results showed that PML had a maximum expression in hESCs and this expression decreased in NPCs and MNCs. Interestingly, three protein bands about 170, 130 and 70 kDa similarly were detected in these cell types on western blots. Conclusion: Based on qRT-PCR results, PML expression may have an important role in cellular pluripotency. However, the appearance of three similar bands in western blots from ESCs, NPCs, and NCs led us to assumption that PML might be necessary in cellular pluripotency and nervous system development.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/401
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