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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۸-۱۰۸
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عنوان انگلیسی Ps-76: Expression Profile of Purinergic Receptors in Human Embryonic Stem Cell and Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cells
چکیده انگلیسی مقاله Objective: Embryonic stem cells (ESCs) and their derivations have significant promising roles in future cell-based therapies. In this regard, many attempts have been performed to differentiate human ESCs (hESCs) to oligodendrocyte progenitor cells (OPCs). OPCs are expected to differentiate to adult myelinating oligodendrocytes in demyelinating disease such as multiple sclerosis. Although these cells are characterized in depth based on their genetic and cell biology, there is limited information about their behavior in response to physiological or pharmacological factors. Among numerous factors that influence different aspects of their development, purinergic signaling is emerging as an important one. Materials and Methods: This study investigated the expression level of different subtypes of purinergic receptors in hESCs and hESC-derived OPCs using quantitative RT-PCR technique to obtain further insight about developmental role of purinergic system in these cells. Results: Our results revealed the expression of A1, A2A, A2B, and A3 adenosine receptors in hESCs. However, hESC-derived OPCs expressed all subtypes of adenosine receptors except for A2A adenosine receptor. Also, we observed several P2X (P2X2, 3, 4, 5, 7) and P2Y (P2Y1, 2, 4, 11, 12, 13, 14) mRNA expression in hESCs. hESC-derived OPCs expressed different subtype Of P2X (P2X1, 2, 3, 4, 5, 7) mRNA and P2Y (P2Y1, 2, 4, 11, 12, 13, 14) mRNA too. Conclusion: This work is the first step to characterize the expression of purinergic receptors in hESCs and hESC-derived OPCs. More studies are necessary to evaluate the physiological role and pharmacological importance of expressed receptors in each stage of cellular differentiation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/402
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