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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۹-۱۰۹
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عنوان انگلیسی Ps-77: Pulsed Electromagnetic Field Enhances SOX9 Gene Expression in Adipose- Derived Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels
چکیده انگلیسی مقاله Objective: Differentiation of mesenchymal stems cells (MSCs) by the means of chemical factors has long being understood. However, the influences of physicalelectromagnetically factors are still under investigation. Further more, the culturing systems of MSCs have a significant role in determining cell fate. As alginate hydrogels support a 3 dimensional niche for cells, they are widely known as a conventional stem cell delivery systemand chondrogenesis specific gene study scaffold. SOX9 is one of the chondrogenic markers that code the SOX9 transcription factor, which plays a critical role in chondrogenesis. In the current study, we tried to assess the ability of extremely low-frequency pulsed electromagnetic field (PEMF) frequency of 25 Hz on the SOX9 gen expression potentials in adipose-derived mesenchymal stem cells (AD-MSCs) encapsulated in alginate beads. Materials and Methods: Rabbit AD-MSCs were obtained from National Cell Bank of Iran (Pasteur Institute of Iran) cultured, at passage 4 they were harvested, and encapsulated in alginate beads. Treatments involved an 8-hour per day exposure to PEMF with the intensity of 1.6 mT, the frequency of 25 Hz, and the duty cycle of 35 ms to 5 ms for a time period of 21 days. Cellular toxicity, histological analysis, and the gene expression of SOX9 were evaluated. Results: MTT analysis demonstrated no toxicity effect of the PEMF applied. Histological analysis showed positive alcian blue staining as a result of glycosaminoglycan synthesis. PEMF treatment resulted in a 608- fold increase in SOX9 gene expression compared to the control group. The present results suggest that PEMF with the defined parameters has the ability to increase the up-regulation of SOX9 gene expression in ADMSCs cultured inalginate constructs. Conclusion: PEMF enhances the deposition of extracellular matrix molecules and augments SOX9 mRNA levels. Optimization of PEMF parameters can improve the chondrogenic potential of AD-MCSs and could be applied for cartilage defects.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/403
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