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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱۰-۱۱۰
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عنوان انگلیسی Ps-79: Laminin Coating Stimulates Polarity, Protein Synthesis, and Metabolism of Hepatocyte-Like Cells Differentiated from Human BM-MSCs
چکیده انگلیسی مقاله Objective: Application of stem cells in cell therapy and tissue engineering for treatment of liver diseases are considered as alternative approach to liver transplantation. So far, extensive studies have been taken to improve hepatogenic differentiation efficiency of different types of stem cells; but not much of interest has been paid to the polarity of the in vitro differentiated cells. The aim of our study was to investigate the effect of laminin on polarity of hepatocyte-like cells differentiated from Human bone marrow (BM)-derived mesenchymal stem cells (BM-MSCs) as well as the protein synthesis and metabolism. Materials and Methods: The characteristics of MSCs obtained from Royan Institute were confirmed by immunophenotyping analysis and their differentiation potential into osteocytes and adipocytes. Hepatogenic differentiation was induced by hepatocyte growth factor, Oncostatin M, and dexamethasone. Undifferentiated human MSCs were used as negative control. On day 21, Immunostaining for albumin (Alb) and alphafetoprotein (AFP) were performed to evaluate the characteristics of the differentiated cells. Furthermore, urea production was evaluated to measure the detoxification capacity of the cells. Finally, F-actin staining was performed to analyze the polarity. Results: The flowcytometry findings revealed that over 90% of the cells expressed the MSC markers but not hematopoietic and leukocyte markers. Also mineralization and oil droplets showed their differentiation potential into osteocytes and adipocytes. The results of immunocytochemistry revealed higher expressions of both AFP and Alb (specific markers of liver) in cells differentiated on laminin matrix compared to that of the control group. Additionally, the results showed that urea production by cells differentiated on laminin was significantly more than that of the polystyrene. Furthermore, few bile canaliculi structures were detected by F-actin staining, in the cells differentiated on polystyrene, while in cells differentiated on laminin the number of detected bile canaliculi was higher. Conclusion: Taken together, these findings may indicate that laminin coating can improve the terminal differentiation of hepatocyte-like cells from human BM-MSCs, by stimulating their protein synthesis, metabolism, and polarity which is fundamental for their function. Thus, laminin might be considered as a suitable coating in hepatic tissue engineering designs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/404
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