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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱۱-۱۱۱
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عنوان انگلیسی Ps-80: Hepatic Differentiation Potential of Menstrual Blood- versus Bone Marrow- Stem Cells
چکیده انگلیسی مقاله Objective: Menstrual blood has been introduced as a simply accessible source for stem cell. The aim of this study was to investigate hepatic differentiation capacity of menstrual blood stem cells (MenSCs) compared to bone marrow-derived stem cells (BMSCs) under different protocols. Materials and Methods: Menstrual blood was collected from healthy females using Diva cup. Bone marrow aspirates were obtained from iliac crests of human donors. Samples were isolated using a combination of density gradient centrifugation and plastic adherence. Differentiation was induced by treating cells with different concentrations of hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in culture media (P1, P2, and P3 protocols). Parallel experiments were carried out to assess expression of hepatic markers at mRNA and protein levels. Furthermore, Glycogen storage and albumin (ALB) secretion were evaluated to find out functionality of differentiated cells. Results: The differential expression of mature hepatocyte markers such as albumin (ALB), cytokeratin 18 (CK-18), tyrosine aminotransferase and cholesterol 7 alpha-hydroxylase activities (CYP7A1) at both mRNA and protein levels in differentiating MenSCs was significantly higher in upper concentration of HGF and OSM (P1) compared to lower concentration of these factors (P2). Moreover, omission of serum during differentiation process (P3) caused typical improvement in functions assigned to hepatocytes in differentiated MenSCs. While up-regulation level of ALB and CYP7A1 was higher in differentiated MenSCs compared to driven BMSCs, expression level of CK-18, detected level of produced ALB and glycogen accumulation were lower or not significantly different. Conclusion: We proved MenSCs have capability to generate functional hepatocyte-like cells, although they showed different expression pattern compared to BMSCs depend on critical growth factor concentration and culture media condition.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/405
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