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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱۲-۱۱۲
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عنوان انگلیسی Ps-81: Three Dimensional Culturing System and Fibroblast Growth Factor-4 Improved Expression of Hepatic Nuclear Factor-4 as A Main Gene in Hepatogenesis
چکیده انگلیسی مقاله Objective: The aim of this study was to find the effect of 3D culture system and FGF-4 on the expression of HNF-4 by HWJMSC which is differentiated toward hepatocyte. The scaffolds and the growth factors are considered as a cell culture support that impact stem cell differentiation. FGF4 is a bioactive protein that has mitogenic activity and induces the cells toward definitive endoderm. HNF 4 has a significant role in the differentiation of the hepatocyte lineage. It was shown that HNF4 secreted from the visceral endoderm and required for the expression of several factors in the liver-specific phenotype through induction of various liver-specific functions Materials and Methods: MSCs derived from Wharton's jelly explants were characterized by flow cytometry. Then, the cells were cultured in the presence of hepatogenic media with or without FGF4 on 2D and 3D collagen scaffold for 21 days. The expression of HNF-4 was evaluated by Real time RT-PCR. Results: The cultures pre-exposed to FGF4 in 3D culture system expressed higher levels of HNF-4, compared to the other cultures. Conclusion: The result showed that both FGF-4 and 3D culture system influence on over expression of HNF-4. 3D culture system mimicked the intracellular microenvironment and improved the hepatic differentiation of human MSCs derived from Wharton's jelly.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/406
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