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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱۶-۱۱۶
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عنوان انگلیسی Ps-85: ALDH1: Is A Useful Biomarker for Identifying Colon Cancer Stem-Like Cells in HT-29?
چکیده انگلیسی مقاله Objective: Recent evidence suggested that epithelial cancers including colorectal cancer (CRC) are driven by a small population of self-renewing, multi-potent cells termed cancer stem cells (CSCs) which could be responsible for the recurrence of cancer. Aldehyde dehydrogenase 1 (ALDH1) activity has been used as a functional stem cell biomarker to isolate CSCs in different cancers such as colorectal cancer. The main aim of this research was to determine the utility of ALDH1 activity along with CD44 and EPCAM in identifying stem cell–like cells in human HT-29 colonic adenocarcinoma cell line. Materials and Methods: In the present study, colon CSCs biomarkers including CD44, EPCAM and ALDH1 were analyzed by flow cytometry in colonospheres and parent cells. The expression levels of stemness genes in spheroid and parental cells were investigated using real-time PCR. In addition, in vivo xenografts assay was performed to determine tumorigenic potential of tumor spheroid cells. Results: According to the results, over 96% of spheroids were CD44+/EpCAM+, while only 37% expressed CD44/EpCAM biomarkers. Controversially, ALDH activity was about 2-fold higher in the parent cells than inspheroid cells. Compared with the parental cells, expression levels of ‘‘stemness’’ genes, like Sox2, Oct4, Nanog, C-myc, and Klf4 were significantly increased in colonosphere cells (p < 0.05). Further, administration of 2500 spheroids could be sufficient to initiate tumor growth in nude mice, while 1x106 of parental cells was needed to form a tumor. Conclusion: It doesn’t seem that ALDH1 would be a useful biomarker to identify CSCs population in HT- 29 cell line. However, colonospheres with low ALDH1 activity indicated increased tumorigenic potential and stemness properties.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/410
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