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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱۷-۱۱۷
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عنوان انگلیسی Ps-86: Identification and Characterization of Cancer Stem Cells in Colon Adenocarcinoma Patients
چکیده انگلیسی مقاله Objective: Proliferation and expansion of cancer stem cells as spheroids were proved in previous studies. But, capability of primary tumor derived cancer stem cells to keep their special properties in vitro is still disputed. So, the goal of this study is to isolate, expand and characterize primary tumor derived colon cancer stem cells Materials and Methods: In the present work, colon CSCs markers including CD44 and EPCAM in spheroid and parental cells were analyzed by flow cytometry. The expression levels of stemness genes in both spheroid and parental cells were investigated using realtime PCR. Tumorigenic potential of spheroid cells was evaluated using in vivo xenografts assay Results: Our data showed 79% of spheroids were CD44+/EpCAM+, while only 20% of parental cells expressed CD44/EpCAM markers. In compared with the parental cells, expression levels of ‘‘stemness’’ genes, like Sox2, Oct4, Nanog, C-myc, and Klf4 were significantly increased in spheroid cells (p< 0.05). Further, As little as 1000 spheroid cells were sufficient to obtain tumor growth in nude mice, while 1x106 of parental cells was needed to form tumor. Conclusion: Sphere formation assay is a useful method for enrich cancer stem cells. Spheroid cells showed increasing expression of stemness genes and tumorigenesis in nude mice.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/411
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