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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۲۳-۱۲۳
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عنوان انگلیسی Ps-92: Assessment of Mvh Expression Following Treatment with Phoenix Dactylifera Date Palm Pollen in Neonate Mouse Spermatogonial Colony
چکیده انگلیسی مقاله Objective: Phoenix dactylifera date palm pollen (DPP) is one of the traditional medicines for male infertility treatment. This study aimed to evaluate the in vitro effects of aqueous extract of DPP on the Mvh (The germ cell specific gene) expression upon neonate mouse spermatogonial stem cells (SSCs) colony formation. Materials and Methods: Cell suspension includes sertoli cells and SSCs were isolated from neonatal 6 day-old mice testes by 2 steps enzymatic digestion and cultured in a DMEM and FCS 4% in the absence or presence of different doses of aqueous extract of DPP (0.06, 0.25 and 0.62 mg/ml) for 2 weeks. The expression of Mvh (VASA) was assessed using Real Time PCR at the end of the culture period. Statistical analysis was determined using ANOVA and paired samples t test at the level of p≤ 0.05. Results: After 2 weeks of culture, there were no significant differences in the expression of Mvh gene between control (0.000064 ± 0.00008), 0.06 mg/ml(5.11 ± 6.86), 0.25 mg/ml (0.0157 ± 0.022) and 0.62 mg/ml concentration (0.000082 ± 0.00004) groups. Conclusion: Our results showed that in the colonies derived from SSCs, the expression of Mvh gene were not significantly increased in the presence of different doses of aqueous extract of DPP.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/417
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