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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۲۵-۱۲۵
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عنوان انگلیسی Ps-94: Effect of Umbilical Cord Blood Derived Platelet-Lysate on Fibroblast Proliferation and Activation of Smad Signaling Pathway
چکیده انگلیسی مقاله Objective: Bioactive substances of platelets play a pivotal role in wound healing. To investigate the mechanisms underlying platelet-induced wound healing using fibroblast, we studied the effect of umbilical cord blood platelet lysate (UCB-PL) on fibroblast, as potent cell in wound healing. In addition, the effect of UCBPL on the activation of smad 2/3 after wound scratching have been studied. Materials and Methods: We prepared platelet extract from cord blood plasma, then (1×105 cells/ml) fibroblast was treated by different concentrations of UCB-PL (5-8-10-15-20%) the mixture of UCB-PL and fetal bovine serum for 24, 48, and 72 hours. Cell viability and proliferation rate were assessed using manually count by trypan blue. Scratch assay was used to evaluate wound healing in vitro were assessed using manually count by trypan blue scratch assay respectively. Then the expression of gene smad2- smad3 was determined by real-time PCR .Then we used western blotting for analysis in order to investigate psmad2/3, smad2/3 signaling pathway. Results: Our results demonstrate an increase in cell proliferation at concentration of 10% of UCB-PL without any effect on cell viability. The same dose of UCBPL induced a significant increase of wound closure rate at 6, 12 and 24 hours after treatment. However it was observed that the increased gene expression ratio in smad3 and smad2 genes at 10% of UCB-PL compare with negative and positive controls significant effect on protein levels on smad3, smad2, psmad2 and psmad3 proteins. Conclusion: UCB-PL can be a satiable supplement for fibroblast culture in vitro and stimulates fibroblast growth and motility which induce wound healing through the activation of different signaling pathways including smad 2/3 signaling pathway in vivo.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/419
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