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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۲۷-۱۲۷
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عنوان انگلیسی Ps-96: Investigation of Effect Condition Medium Derived from Cultured Pancreas on Differentiation of Insulin Secreting Cells from Stem Cells
چکیده انگلیسی مقاله Objective: About 150 millions people are affected to diabetes mellitus through the world. Recent studies have shown that many types of stem cells can be possible sources to derive transplantable insulin-producing cells (IPCs). The aim of the present study was to investigate the effects of pancreas conditioned medium on production of IPCs from undifferentiated Embryonal carcinoma cells (EC). Materials and Methods: In this study, the c.m of cultured pancreas from one-week newborn mouse was used for differentiation of P19. EBs formed by a 24 hour suspension culture of P19 EC cells. In order to induce the difrentiation, different concentrations of c.m (25, 50, 75 and 100%) were added to culture medium. Dithizone staining was used for the detection of ECderived IPCs. Insulinproinsulin production and insulin receptor beta were determined by immunofluorescence. The expression of pdx-1 gene was also analyzed by reverse transcriptase-polymerase chain reaction. Data were analyzed using one way ANOVA and Dunkana test. Results: Differentiated cell clusters appeared after approximately 7 days induction. The peak response of differentiation was at the concentration of 50% c.m. The differentiated cells were immunoreactive for β cell-specific proteins, insulin-proinsulin and β receptor. RT-PCR showed the expression of a pancreatic β cellrelated gene, Pdx-1. The data presented showed that it is possible to generate IPCs from undifferentiated EC cells with the characteristics of pancreatic β cells. Conclusion: A major obstacle in Pancreatic islet transplantation is the inadequacy of pancreatic islets. Many studies have done to expand pancreatic islets and to develop renewable sources of islet-replacement tissue. EC stem cells are developmentaly pluripotent cells which can be differentiated into all cell types under the appropriate conditions.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/421
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