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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۲۸-۱۲۸
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عنوان انگلیسی Ps-97: Detection of Mycoplasma Contamination in Hair Follicle Stem Cells Culture
چکیده انگلیسی مقاله Objective: Stem cells such as hair follicle stem cells are important for research and cell therapy. Nestin-expressing stem cells have been previously shown to be pluripotent and be able to form neurons and other nonfollicle cell types. One of the aims of this study was to detect frequent mycoplasma species in hair follicle stem cells culture (M. hyorhinis and M. arginini) with culture and PCR methods. Materials and Methods: In this study young mouse hair follicle, human fetal shoan stem cells, Hela and vero cell lines were used for detection of mycoplasma. First, the stem cells and cell lines were cultured for 14 days after thawing, and supernatant of these cultures were taken after a culture period of at least 5 days. Then, supernatant of these cell cultures were used for samples and were divided into 2 sections: first section was passed through 0.45 μm pore-size filters, then inoculated into specific PPLO broth and agar media and incubated at 37°C under 5% CO2 in 7 days . Culture method were only used for detection of M. arginini because M. hyorhinis is non-cultivable. The second section was used for PCR in which primers were used for amplification of 16S rRNA gene of M. hyorhinis and M. arginini. Results: From a total of 8 samples tested, 0% were positive with culture and with PCR method for M. arginini and also 0% were positive with PCR method for M. hyorhinis. Conclusion: Results of this study showed that no contamination in cell lines especially in stem cells with culture and PCR methods. Since good practice in the laboratory and the frequent monitoring of the stem cell lines is important for this research.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/422
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