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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۲۹-۱۲۹
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عنوان انگلیسی Ps-98: Liver Fibrosis Treatment Using Secreta of Bone Marrow Mesenchymal Stem Cells in Rat
چکیده انگلیسی مقاله Objective: Mesenchymal stem cells therapy may prevent parenchymal cell loss and promotes tissue repair through the action of trophic secreted molecules. This study evaluated the hypothesis that infusions of secreta (conditioned culture medium) of bone marrow mesenchymal stem cell can improve liver fibrosis in an experimental rat model. Materials and Methods: Eighteen rats with induced liver fibrosis using thioacetamide (30 intraperitoneal injections biweekly) were divided into three groups. Two groups were intraperitoneally injected with secreta of bone marrow mesenchymal stem cells (1 ml of culture medium of 90% cell confidence without cell) 20 days after the last thioacetamide injection. They were euthanized 4 and 6 weeks after transplantation. The last group was selected as control and was euthanized 20 days after the last thioacetamide injection. Liver samples were histopathologically evaluated using Hematoxylin and Eosin staining and Masson’s trichrome staining. Serum alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase and albumin concentrations were compared between groups. Serum parameters were evaluated using one-way ANOVA. Histopathologic scores were analyzed using Mann-Whitney test (SPSS 11.5). P< 0.05 was considered significant. Results: Injection of secreta of bone marrow mesenchymal stem cells improved survival, liver fibrosis and necrosis in rats with thioacetamide-induced liver fibrosis. Secreta of bone marrow-derived mesenchymal stem cells also significantly recovered serum concentrations of alanine aminotransferase after 4 and 6 weeks (93.83 ± 3.42 U/L and 73.40 ± 2.65 U/L, respectively) and alkaline phosphatase after 6 weeks (281.80 ± 39.53 U/L) in comparison with control (132.0 ± 17.95 U/L and 488.33 ± 89.05 U/L, respectively). Conclusion: Bone marrow mesenchymal stem cell secreta exosomes can relieve thioacetamide-induced liver fibrosis. This provides a novel approach for the treatment of fibrotic liver disease.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/423
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