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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۳۰-۱۳۰
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عنوان انگلیسی Ps-99: The Effect of Substrate Elasticity on Cell Adhesion Force of Endothelial Cells
چکیده انگلیسی مقاله Objective: Cells have dynamic structures with various functions, such as adhesion, migration, and signal transduction which can be dramatically influenced by their extracellular environment. Cells respond to their environmental cues including chemical, topological, and mechanical signals to maintain homeostasis for their function as well as their integrity. The cellular responses are in accordance with stimuli transmitted by adhesion protein receptors which create intracellular signaling resulting in stronger adhesions of the contact cell or cell motility. Many pathological diseases can be caused by abnormal cellular or tissue mechanics and mechanotransduction caused by environmental change. Materials and Methods: In this study, we used Poly acryl amid (PAA) to create three substrates with different stiffness in order to see the effect of substrate elasticity on cell adhesion force. Human umbilical vein endothelial cells (HUVEC) obtained from National Cell Bank of Iran (Pasteur Institute of Iran) were used to determine the toxicity of substrates using the extracts through MTT assay. Surface topology and elastic moduli of substrates were assessed by means of Atomic Force Microscopy (AFM). Cell-substrate adhesion force of endothelial cells on different substrates was estimated using Single Cell Force Spectroscopy (SCFS). Results: MTT results showed more than 90% cell viability for all three samples. Endothelial cells were attached more tightly to the substrate with the highest stiffness while cells are more spherical-like on the softest substrate. Measured cell-substrate adhesion force demonstrated a significant increase from a soft substrate to a hard one that is 328, 418.8 and 783 nN on soft, medium, and hard substrates respectively. The results of topography depicted the similar topography of three different surfaces. Conclusion: The results of this study revealed that cell adhesion is actively responding to any changes in the mechanical properties of its extracellular matrix. These findings emphasize the importance of substrate stiffness in addition to other cues which can be used in differentiation to a special cell type and tissue engineering applications.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/424
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