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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۳۳-۱۳۳
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عنوان انگلیسی Ps-102: H2O2 as A Preconditioning Agent Increases Mesenchymal Stem Cells Survival: An In Vitro Study
چکیده انگلیسی مقاله Objective: A growing number of experimental studies highlight the potential of stem cell transplantation as a novel therapeutic approach for diseases. But the massive loss of stem cells post-engraftment is an impediment that lessens the effectiveness of cell transplantation therapy. The present study tried to increase the survival of the stem cells by preconditioning them with H2O2. Materials and Methods: Mesenchymal Stem cells were isolated from rat bone marrow. MSCs from passage four were cultured containing different concentrations of H2O2 (0, 5, 10, 20, 30, 40, .80, 90, and 100 μM) for 6, 12, 24, and 48 hours and then were recovered with fresh medium. Finally treated cells were exposed once to a lethal dose of H2O2 (300μM and 500μM).The viability of the cells were evaluated with trypan blue and proliferation assay with MTT assay. Results: Preconditioning with 5μm H2O2 significantly increased the resistance of these cells against lethal conditions induced by H2O2 (p< 0.05). MTT assay showed higher proliferation in groups treated with 5 and 10 μM H2O2 after 24 hours. Conclusion: Preconditioning of MSCs with oxidative stresses enhances their survival and could therefore increase the efficacy of MSCs transplantation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/427
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