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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۳۶-۱۳۶
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عنوان انگلیسی Ps-105: Assessment of α6 and β1 Integrins Expression after Continuous and Pulsed Low Intensity Ultrasound on Mouse Spermatogonial Stem Cells
چکیده انگلیسی مقاله Objective: Low intensity ultrasound (continuous and pulsed) is a form of energy. Spermatogonial stem cells (SSCs) maintain spermatogenesis throughout the life of the male. This study investigates the effects of low intensity ultrasound stimulation (LIUS) and Low Intensity Pulsed Ultrasound Stimulation (LIUPS) on the expression of germ cell-specific and pluripotency genes on SSCs during in vitro culture. Materials and Methods: Isolated SSCs from neonatal mice were cultured in DMEM culture medium with 10% Fetal Bovine Serum (FBS). Then to confirm identification of SSCs, PLZF protein was detected in these cells and SSCs derived colonies. SSCs were stimulated by LIUS and LIUPS for 5 days then expression of α6 and β1 integrins (germ cell-specific gene) and Oct-4 genes (pluripotency gene) were assessed at day 21 and were analyzed by RT-qPCR. Data analysis was done with ANOVA test. Results: The LIUS and LIUPS treatment of mouse SSCs increased the expression of α6 and β1 integrins genes in experimental groups compared to the control group (p< 0.05), whereas there were not significant differences between groups regarding to expression of Oct-4 gene. Conclusion: These results suggested that LIUS and LIUPS treatment had the good effect based on genespecific marker expression.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/430
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