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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۳۸-۱۳۸
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عنوان انگلیسی Ps-107: Mathematical Modelling of Dynamic Culture in The Perfusion Bioreactor on A Three-Dimentional Model of Bone Formation
چکیده انگلیسی مقاله Objective: The medium flow in the construct is essential for mammalian cells to grow and distribute uniformly in three-dimensional (3D) construct due to their specific nutrients’ needs. Dynamic culture improves the distribution of nutrients and mass exchange which promotes the cell growth. Due to the complex and multiphase microstructure in the process, development of models for capturing the aforementioned knowledge is studied. The aim of this work was, the study of the effect of nutrient flow on cell growth in 3D scaffold. Materials and Methods: A first the bone cells (designation: G292, species: human, national cell bank, Pasteur Institute, Iran) were cultured on a 3D sponge collagen scaffold (Matricel Company,Germany) for 24 hours in static culture and then the cultured scaffold was placed in a perfusion bioreactor with flow rate of 0.04 ml/minutes for 21 days. The scaffold was weighed in first and 21st days and the weight of the original scaffold was deducted from these weights. So, the ratio of cell density was obtained. The mathematical model proposed in this article included Brinkman, nutrient transport and cell growth equations and also permeability variation in the construct due to cell growth. Finally, the model was compared to the result of the experiment. Results: As the result of the experiment showed, cell density was 53.5 times more numerous after 21 days; compared to the results of the model, it was 49 times. Consequently, the model has been almost validated. Conclusion: This work has developed a mathematical model successfully that simulates the effect of nutrient flow on bone cell growth. The proposed model can be used for other dynamic culture to predict the amount and distribution of cell growth in 3D scaffold.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/432
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