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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۱-۱۴۱
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عنوان انگلیسی Ps-110: Neural Stimulation of Human Hair Follicle Dermal Papilla Stem Cells by Extremely Low Frequency Electromagnetic Field
چکیده انگلیسی مقاله Objective: Chemical factors are among the most frequent signaling factors employed for stem cells differentiation into neural cells. Effect of physical factors on cell behavior recently has been proven. The aim of this study was to illustrate the effect of electromagnetic (EM) signaling on the differentiation of human hair follicle dermal papilla stem cells (DPCs) into neural cell by the evaluation of nestin (progenitor neural cells marker) and MAP2 (mature neuron marker) genes. Materials and Methods: This study was designed to evaluate the neural markers' gene expression after 5 consecutive days treatment in 4 treatment groups defined as control, chemical, EM, and chemical-EM. To assess the role of extended treatment timing on neural differentiation markers m RNA levels, an EM group was prepared, treated for a time period of 14 days, and compared with a control group. In all groups, DPCs were cultured on 0.1% gelatin coated flasks. In the chemical tests, DPCs were cultured in DMEM medium containing EGF, bFGF, and DMSO while in the EM group, cells were cultured in DMEM medium and were exposed to EM field (1 mT, 50 Hz, 7hours/day). In the chemical-EM treatment group, DPCs were subjected to EM field together with chemical induction medium. After the treatment period, real-time PCR method was used to study the gene expression of nestin and MAP2. Results: According to gene expression analysis, nestin gene expression levels in chemically stimulated group was shown to be 0.8265 fold, a 1.366 fold increase for EM (5d) treated group, a 3.9665 fold increase for the chemical-EM group and the EM (14d) treatment group showed a 1.7178 fold increase compared to the control group. Chemical induction generated 5.247 fold increase in MAP2 mRNA levels, whereas EM (5d) did not show any significant increase, no gene expression was detected for chemical-EM treated group and a 2.545 fold increase was observed in EM (14d). Conclusion: Results from the chemically stimulated group are indicative of a high differentiation rate of DPC to mature neuron. The comparison between EM (5d) and EM (14d) results indicates that EM (5d) is at an early stage of differentiation. In EM (14d) group, the expression of MAP2 experienced a significant increase (2.545 fold) compared to a previous study (similar EMF parameters, but a 12 days continuous treatment). Lack of MAP2 gene expression in chemical-EM group seems to be attributed to the increase in intracellular reactive oxygen species (ROS) that leads to apoptosis. It has been shown that both epidermal growth factor and electromagnetic field increase ROS levels, however as progenitor neural cells have high level capacity of ROS, the significant increase in nestin expression in this group can be related to a cellular survival trend.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/435
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