این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۳-۱۴۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-112: Using Western Blotting Technique to Study The Amount of MAP-2 and Nestin Proteins in Neural Differentiated Human Adipose Derived Stem Cells
چکیده انگلیسی مقاله Objective: Human adult stem cells which are capable of self-renewal and differentiation into other cell types, can be isolated from various tissues. There are no ethical and rejection problems as in the case of embryonic stem cells, so they are a promising source for cell therapy. The human body contains a great amount of adipose tissue which contains high number of mesenchymal stem cells (MSCs) that can be easily obtained in large quantities by a less invasive method in comparison to bone marrow-derived MSCs. The aim of this study was to isolate, characterize and explore the potential of human adipose derived stem cells (hADSCs) in differentiating toward neural lineage. Materials and Methods: To isolate ADSCs, adipose tissue was digested with collagenaseI and cultured in DMEM: F12/10% FBS. The expression profiles of several MSC markers were examined by flow cytometry. After neurosphere formation, final differentiation was induced by RA and BDNF. Differentiated ADSCs were evaluated for Nestin and MAP-2 proteins expression by using western blotting technique. Results: MSC markers identified by flow cytometry in isolated ADSCs. Our results show that these cells could be differentiate into neuron-like cells as defined by cell morphology and expression of neural proteins (MAP-2 and Nestin) in compare with undifferentiated cells. Conclusion: The present study demonstrated that we were succeeded in induction of ADSCs toward neural lineage and the expression levels of MAP-2 and Nestin proteins were increased.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/437
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات