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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۵-۱۴۵
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عنوان انگلیسی Ps-114: Assessment of Human Embryonic Stem Cell Derived Neural Progenitor Cells Behavior on Nanofibrous Conductive Substrates
چکیده انگلیسی مقاله Objective: Recent research has provided evidence that neural progenitor cells can sense their physical environment as they do in the case of chemical cues. The cell response to these physical properties such as stiffness, topography, electrical conductivity, etc would be considered as accelerated or reduced cell proliferation, differentiation toward a specific cell lineage and better in vitro functionality. In present study we will assess NPCs response to surface nanotopoghraphy and conductivity. Materials and Methods: To achieve the above objective we used graphene and nano polycaprolactone (PCL) coated cover slips produced in Royan institute. Our experimental groups are graphene, polycaprolactone nanofibers and both graphene and PCL nanofibers compared with none coated simple cover slips. Results: Primary results revealed undisputed cell alignment with nanofibers. Compared with O4+ and GFAP+ cells, higher population of TUJ1+ cells was observed in immunostaining. Despite identical seeding cell population for all groups, in nanofibers and nanofiber+ graphene coated plates higher number of cell were observed due to more proliferation or better cell survival. In the ongoing tests we are to prove which type of neuron is produced and check the impact of surface conductivity on functionality of produced neuron. Conclusion: Cell alignment with nanofibers could help guided axonal growth in regenerative cell transplantation strategies. Considering higher population of TUJ1+ cells,it can be concluded that NSCs had more tendency to differentiate to neurons rather than Oligodendrocytes and astrocytes.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/439
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