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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۷-۱۴۷
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عنوان انگلیسی Ps-116: Generation and Characterization of Human Induced Pluripotent Stem Cells Derived from Diabetic Patient Using Polycistronic SOKM Non-Integrating Lentiviral System
چکیده انگلیسی مقاله Objective: One of the promising approaches to understand and cure diabetes is to use pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Human iPS cells can potentially serve as a source for cell replacement therapy without the ethical obstacle of embryonic stem cells and would bypass immune rejection. Obtaining hiPS cells genetically identical to the patient presenting with diabetes has been a longstanding dream for the in vitro modeling of disease and ultimately cell therapy. Materials and Methods: Induced pluripotent stem (iPS) cells were generated from Iranian diabetic patient by reprogramming their adult fibroblasts with four defined factors (Sox2, Oct4, Klf4, c-Myc) with the prospect of differentiating into insulin-producing cells to apply in cell replacement therapy. Following picking up the iPS-like colonies, confirmation tests including alkaline phosphatase staining, RT-PCR, real-time PCR, immunocytochemistry, embryoid body formation and in vitro differentiation were carried out. Results: Fourteen days post infection of fibroblasts, hiPS colonies were picked. These cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens and gene expression which were indicated by high expression level of Alkaline Phosphatase, Nanog, SSEA4 and OCT4. Conclusion: These iPS cells were shown to be similar to embryonic stem cells therefore having great potential for the use in regenerative medicine and for modeling genetic diseases.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/441
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