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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۸-۱۴۸
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عنوان انگلیسی Ps-117: Increase of GAG Secretion from MSCs in Response to The Functional Group Interactions of Amphiphilic Scaffolds
چکیده انگلیسی مقاله Objective: One of the key challenges for any cartilage tissue engineering approach is providing adequate functional properties so that the engineered construct can mimic extracellular matrix (ECM) components of cartilage. This study focuses on determining the effect of the composition and cross linking of amphiphilic scaffolds on the glycosaminoglycan (GAG) secretion of mesenchymal stem cells (MSCs). Materials and Methods: Scaffold preparation was based on mixing of aqueous and non-aqueous polymer solutions of polycaprolactone (PCL), polyvinyl alcohol (PVA), and gelatin (GEL) followed by homogenizing and freeze-drying. Then, the scaffolds were characterized by fourier transform infrared spectroscopy, and biological assays of MSCs culture, acridine orange/propidium iodide (AO/PI), and dimethyl methylene blue (DMMB) assay. Results: The results from DMMB assay for GAG measurement revealed a significant increase in proteoglycan content for GEL/PCL/PVA scaffolds from 13.3905 ± 1.0429 μg/ml to 16.1767 ± 0.9917 μg/ml after 21 days. AO/PI results showed not only more than 90% of the cells remained alive inside the scaffolds but addition of GEL led to an increase in the number of the alive cells. More MSCs proliferated in the amphiphilic ternary scaffold containing GEL shows that the interactions between functional groups of GEL and PVA in cross linking process can provide a stable biomimetic microenvironment which stimulates MSCs for GAG secretion in cartilage tissue engineering. Conclusion: The study revealed that the functional group interactions in cross linked GEL/PCL/PVA scaffold can increase the GAG secretion of MSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/442
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