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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۴۹-۱۴۹
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عنوان انگلیسی Ps-118: The Effects of Pre- and Post- Ischemic Intra-Ventricle Injections of Mesenchymal Stem Cells on Brain Stroke in Rats
چکیده انگلیسی مقاله Objective: Stroke is the third cause of death and first cause of disability around the world. Stroke could be resulted in systemic and focal inflammation in the ischemic area and it is responsible for an important part of neuronal loss. Expression of inflammatory cytokines, disrupting blood brain barrier, exposing central nervous system to peripheral immune system, up regulating of adhesion molecules are some results of inflammation after brain ischemia. Mesenchymal stem cells(MSCs) are one of the stem cells categories that could be isolated from different tissue such as adipose tissue, bone marrow, etc. MSCs are capable to reduce inflammation by secreting some anti-inflammatory cytokines such as IL-10. MSCs can also regulate immune system. Therefore the aim of this project was to evaluate effects of intra-ventricular injection of MSCs on stroke in rats. Materials and Methods: MSCs were obtained from fresh adipose tissue of the rats’ abdomen after adding collagenase. Their multi-potency was confirmed by differentiating into adipocyte and osteocyte. The MSCs labeled with PKH26 and injected into lateral ventricle by stereotactic method before (pre-ishchemic group) and after (post-ischemic group) induction of stroke. The control group received PBS. Neurological assessments were done daily until 28 days. After fixation and sectioning, the brains were stained by HandE to determine the necrosis and ischemic area. Results: The neurological assessment showed that clinical improvement in post-ischemic group was better than pre-ischemic and control groups after 28 days. Tracking of the Mesenchymal stem cells by PKH26 showed that the labelled MSCs migrate to cortex and striatum more than other areas in both treatment groups. HandE staining also showed that post-ischemic and control groups had the lowest and highest necrosis and ischemic areas, respectively. Conclusion: Post-ischemic MSCs stem cell injection improved neurological signs and reduced ischemic and necrosis area more than pre-ischemic injection.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/443
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