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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۵۰-۱۵۰
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عنوان انگلیسی Ps-119: Preconditioning of Bone Marrow– Derived Mesenchymal Stem Cells with H2O2 Improved Regenerative Potential of MSCs in Mice Liver Acute Injury
چکیده انگلیسی مقاله Objective: Bone marrow–derived mesenchymal stem cells (BM-MSCs) are attractive cell sources for cell therapy. However, the efficacy of MSCs is limited because of low survival rate following translation. In this study, we preconditioned MSCs with sub-lethal doses of H2O2. We next examined the effects of preconditioning on the therapeutic potential of MSCs transplantated to acute liver injury in mice. Materials and Methods: MSCs were isolated and cultured in low DMEM. Cells from passage four were plated at a density of 15000 cells/cm2 in 96-well plate and treated with 5, 10, 15, 20, 30, 40, 50, 60, 80, and 100μm concentrations of H2O2 (24 hours), and then were recovered in fresh medium for 7 hours followed by exposure to lethal doses of H2O2. Cell viability was measured with WST assay. Next, in the in vivo phase, we first induced acute liver injury then evaluated regenerative potential of conditioned MSCs. 48, 72, 96, and 120 hours later. Blood and liver samples were collected and analyzed using biochemical as well as histological methods. Results: In the groups engrafted with pre-conditioned MSCs, survival rates was higher in comparison to who received normal MSCs. ALT and AST reached to normal level in the groups engrafted with pre-conditioned MSCs. Intrestingly, histological results revealed a significant improvement in liver regeneration potential in the groups transplanted with pre-conditioned MSCs. Conclusion: Preconditioning of MSCs with oxidative stresses not only enhances their survival in vitro, but also increases the efficacy of MSC-based cell therapy in liver acute injury.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/444
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