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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۵۶-۱۵۶
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عنوان انگلیسی Ps-125: Effect of Chromium Chloride on Adipogenic Differentiation in Mouse Bone Marrow Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Bone marrow mesenchymal stem cells (BMSCS) are multipotent, and proliferate freely in vitro to undergo self-renewal and differentiation into multiple non-hematopoietic cell lineages such as adipocyte. Adipogenesis is a complex process that includes the proliferation of precursor cells, their commitment to the adipogenic lineage and terminal differentiation. Adipocytes deriving from MSCs in vivo, play a critical role in both normal physiology and the progression of various disease states. Chromium (cr3+) is an essential micro-nutrient and biochemical trace element in physiological functions. The aim of the present study was to evaluate the effects of different concentrations of chromium chloride on adipogenic differentiation in mouse bone marrow mesenchymal stem cells. Materials and Methods: In the present study, mouse mesenchymal stem cells were isolated from an aspirate of bone marrow harvested from the tibia and femoral marrow compartment. Cells cultured in DMEM culture medium supplemented with 5٪ FBS for 72 hours. After the first passage cells were cultured in differential medium in group I (0.01 mg/ml insulin) and group II (0.01mg/ml insulin and 400 ng/ml dexamethasone) with different concentrations (0, 5, 10, 25 and 50μM) of chromium chloride (control and treatments I, II, III, IV and V, respectively) Cells were cultured for 21 days. The cell viability was assessed and adipose droplets formation was observed under light microscope. Sudan III and PAS staining were performed for detection of lipid and carbohydrate in the cells. Results: Our results showed that the highest viability was in treatment I and the lowest viability was in treatment V in groups I and II. Microscopic observation established the formation of lipid droplets and carbohydrate storage in all treatments of both groups but intensity of stain was higher in group II, treatments IV, V. Conclusion: Based on these observations, we conclude that the viability of cells in treatments reduced according to increasing the concentration of chromium chloride and with passing time.The result showed thatwith the use of chromium chloride in medium at least can increase cell differentiation into adipocyte.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/450
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