این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۶۲-۱۶۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-131: Comparison of The Ability of Differentiation of Adipose Derived Stem Cells (ASC) to Osteocytes after Using Different Types of Composites Including Polyvinyl Alcohol-Gelatin-Hydroxy Apatite, -SiO2, -ZrO2 and Gelatin
چکیده انگلیسی مقاله Objective: The management of large bone defects because of serious trauma, cancer, or congenital abnormalities is one of the major orthopedics problems. Tissue engineering can replace the damaged tissues using biologically compatible scaffolds along with suitable stem cells. The aim of this study was to compare the effect of different types of composites including Polyvinyl alcohol-Gelatin-hydroxy Apatite, -SiO2, -ZrO2 and pure gelatin on the differentiation potential of adipose derived stem cells (ASCs) to osteocytes. Materials and Methods: The scaffolds were fabricated by electrospinning. Cell cytotoxicity and increase or decrease in cell number on different scaffolds was examined by MTT assay after 3 and 7 days post culture. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) level as an early differentiation marker after 21 days at the end of mineralization period. Results: MTT assay showed that ASCs were able to proliferate in all considered scaffolds. Polyvinyl alcohol- Gelatin-hydroxy Apatite and Polyvinyl alcohol- Gelatin-Sio2 had the most and the least cytotoxic effects, respectively. Polyvinyl alcohol-Gelatin-hydroxy Apatite caused 23 and 33% decrease in cell viability at days 3 and 7, respectively. The osteogenesis assays indicated the superiority of Polyvinyl alcohol-Gelatin-Sio2 in supporting ASCs for bone differentiation according to augmentation of cellular ALP levels. Conclusion: The results of this investigation introduce Polyvinyl alcohol-Gelatin-Sio2 scaffold as an appropriate candidate for bone generation in regenerative medicine.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/456
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات