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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۶۷-۱۶۷
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عنوان انگلیسی Ps-136: Mechanical Vibration as A Physical Means to Influence Stem Cell Fate, Promoting Bone-Specific Gene Expression In Vitro
چکیده انگلیسی مقاله Objective: Cells can sense and actively respond to mechanical stimuli. However, such active response promises a large potential in stem cells for future work in therapeutics tissue engineering. Recent studies introduce mechanical vibrations as a new mechanism in stimulating mesenchymal stem cells (MSCs). In the current study we tried to assess the effect of mechanical vibration on the bone-specific gene expression potential of MSCs in vitro. Materials and Methods: To evaluate the effect of vibration loading on MSCs, the mechanical vibration device was designed and implemented at the Amirkabir University of Technology and has been patented in Iran. Bone marrow mesenchymal stem cells (BMSCs) were obtained from National Cell Bank of Iran. Cells were cultured in basal medium and were then subjected to vertical mechanical vibration at the frequency of 300 Hz, set for 45 minutes per day and for the extended period of 21 days. In order to quantify the osteogenic markers of the treated cells, gene expression of Runx2, and ColI was evaluated using a real-time PCR. Control cells were cultured similarly without vibration. Results: RUNX2 is amongst the genes expressed during the early phase of osteogenic differentiation of MSCs. Results indicate that the vibration regime loading of 300 Hz increases the level of RUNX2 2.1 fold more than control group. COLI has a more effective role in the middle stage of differentiation and the vibration regime loading of 300 Hz increases the level of COLI 9.28 fold more than control group. Conclusion: Our results are consistent with previous researches that introduce mechanical vibrations as a mechanism for mechanical stimulation of MSCs. This mechanical stimulus influences both the transcription and the production of extracellular matrix protein levels. Future clinical trials could benefit from the rapid differentiation of mesenchymal stem cells treated with non-invasive mechanical vibration.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/461
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