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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۶۹-۱۶۹
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عنوان انگلیسی Ps-138: The Study of Differentiation of Nurr1, Pitx3-Expressing Mouse Induced Pluripotent Stem Cells (iPSCs) into Dopaminergic- Like Cells
چکیده انگلیسی مقاله Objective: Induced pluripotent stem cells (iPSCs) are considered to be potentially able to differentiate into all human cell lineages and thus hold promise as an unlimited source for cell replacement therapies in clinical applications. Parkinsons disease (PD) is a neurodegenerative disorder in which the dopaminergic neurons selectively lost. Due to the side effects of a dopamine precursor drug, cell replacement therapy especially using pluripotent stem cells and iPS cells for PD treatment has been considered recently. Materials and Methods: In this study we established a mouse induced pluripotent stem cell (iPS) line co-expressed Nurr1 and Pitx3 transcription factors in order to efficiently generate DAergic neurons. Five stage protocol introduced by McKay's laboratory was carried out with some modifications for derivation of DAergic like cell. Expression Level of DAergic-specific markers including TH, MAP2 and NESTIN were analyzed by real time PCR and Immunocytochemistry and measuring of dopamine synthesis as well as its secretion by reverse-HPLC. Results: The results indicated increase in the expression level of DAergic-specific markers including TH, MAP2 and NESTIN. Conclusion: We report here that iPS cells can efficiently generate midbrain precursors and dopamine neurons. The result of this study may have impact on future stem cell therapy of PD.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/463
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