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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۰-۱۷۰
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عنوان انگلیسی Ps-139: The Efficacy of Differentiation Potential of The Breast Milk-Derived Stem Cells into Hepatocyte, Cardiomyocyte, Neuron, Osteocyte and Adipocyte
چکیده انگلیسی مقاله Objective: The human mammary gland as a dynamic organ that undergoes significant developmental changes contains mammary stem cells that are responsible for the changes during pregnancy, lactation and involution. Recently human breast milk can be considered as a novel non-invasive source of stem cells without any ethical controversy. The aim of this study was to evaluate the proliferation and differentiation potential of human breast milk stem cells (hBSCs) into hepatocyte, cardiomyocyte, osteocyte, adipocyte and neuron cell. Materials and Methods: Human milk samples were collected in sterile tubes manually. The breast milk was diluted 1:2 with DMEM medium, centrifuged at 300g for 20 min. The cell pellet was washed twice with PBS containing 7% FBS. The cells were characterized for mesenchymal stem cell CD-markers by flow cytometry. To examine the pluripotency of human breast milk to three germ lineages, hBSCs were exposed to the hepatogenic, myogenic, osteogenic, adipogenic and neurogenic media. The immunofluorescence was performed to verify the differentiation of the hBSCs into various cell lineages. Results: The cells expressed CD44 (88.3 ± 4.3%), CD45 (91.45 ± 506%), CD90 (41.6 ± 0.4%), CD271 (81.2 ± 5.8%), CD106 (9.5 ± 1.4%) and showed a negative reaction for CD34 (2.7 ± 2.1%), CD73 (3.8 ± 0.51%), CD105 (2.64 ± 0.55%) and CD144 (3.9% ± 0.95). The cells exposed to the hepatogenic media expressed CK19, CK18, alpha-fetoprotein and albumin. The myogenic-treated cells also reacted with troponin. The cells treated with osteogenic media were stained with alizarin S; however, the cells showed a weak potential for osteognic fate. The adipogenic-exposed cells were stained with oil red and the cells treated with neurogenic media expressed β-tubulin. Conclusion: The hBSCs showed the potential to differentiate toward the derivative of three germ layers. Therefore, human breast milk can be considered as a non-invasive source of autologous source for cell therapy purposes.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/464
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