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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۳-۱۷۳
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عنوان انگلیسی Ps-142: The Effect of MiR-186 on Insulin Producing Cells
چکیده انگلیسی مقاله Objective: Development of renewable sources of islet-replacement tissue for treatment of type I diabetes mellitus is an interesting worldwide issue. Placental tissue derived mesenchymal stem cells (MSCs) are a great promising source for regenerative medicine due to their plasticity, and easy availability. MicroRNAs (miRNAs) are a class of small non-coding RNAs that play a central role in control of many biological activities. The miR- 186 is expressed in the pancreas and is involved in islet development. Materials and Methods: Human placental decidua basalis (PDB-MSCs) cells were cultured from full term human placenta. The imunophenotype of isolated cells was checked for CD90, CD105, CD44, CD133 and CD34 markers. The MSCs (P3) were chemically transfected with hsa-miR-186. Total RNA was extracted on the fourth and sixth day after transfection. The expressions of insulin, NGN3, PAX6, KIR6.2, PDX1 and Glucagon genes were evaluated by real- time qPCR on the sixth day. Results: Flow cytometry analysis confirmed that more than 90% of cells are CD90+, CD105+, CD44+ and negative for CD133 and CD34. Morphological changes were followed from the second day, and during the sixth day cell clusters were formed.The expression of pancreatic specific transcription factors were remarkably increased during the four days after transfection and significantly increased on the seventh day. Conclusion: The MSCs could be programmed into functional insulin producing cells by transfection of miR-186.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/467
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