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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۴-۱۷۴
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عنوان انگلیسی Ps-143: The Effect of MiR-375 on Induced Pluripotent Stem Cells toward Islet-Like Cell Clusters
چکیده انگلیسی مقاله Objective: Diabetes mellitus is characterized by either the inability to produce insulin or insensitivity to insulin secreted by the body. Islet cell replacement is an effective approach for diabetes treatment; however, it is not sufficient for all the diabetic patients. MicroRNAs (miRNAs) are a class of small non-coding RNAs that play an important role in mediating a broad and expanding range of biological activities, such as pancreas development. The present study aimed to develop a protocol to efficiently differentiate human induced pluripotent stem cells (IPS) into islet-like cell clusters (ILCs) in vitro by using miR-375. Materials and Methods: The human iPS colonies were transfected with hsa-miR-375 by using siPORT™ NeoFX™ Transfection Agent. Total RNA was extracted 24 and 48 hours after transfection. The gene expressions of insulin, NGN3, GLUT2, PAX4, KIR6.2, PDX1, and OCT4 were then evaluated through Real- time qPCR. Results: Morphological changes were observed on the first day after the chemical transfection and cell clusters were formed on the third day. The expression of pancreatic specific transcription factors was increased on the first day and they had significantly increased on the second day. The ILCs were positive for insulin and NGN3 proteins in the immunocytochemistry. Conclusion: Overexpression of miR-375 can be an alternative strategy for producing ILCs from the iPS cells in a short time.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/468
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