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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۵-۱۷۵
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عنوان انگلیسی Ps-144: Embryonic Stem Cells-Derived Cardiomyocytes in Suspension Culture as A Good Model for Evaluation of Doxorubicin Cardiotoxicity
چکیده انگلیسی مقاله Objective: Nowadays highly efficient production of cardiomyocytes from human pluripotent stem cells is one of the most important subjects in stem cell research. These cardiomyocytes can be used for identifying developmental pathways, disease modeling, drug screening, and are ultimately used in clinical phases. So far, several protocols have been utilized for human embryonic stem cells (hESCs) differentiation into cardiomyocytes in vitro. Materials and Methods: In the present study, it was tried to differentiate spheres generated from hESCs into cardiomyocytes in a define medium using small molecules. The physiology of mentioned cells has been evaluated by MTS assay for viability determination, flow cytometery analysis of DCFDH for ROS assessment, and morphological study using histologic microsections, in response to different doses of doxorubicin (0.03, 0.3, 3, 30μM), an effective anti-cancer drug- with cardiotoxicity side, effect. Results: Results of the study showed that at doses of 3 and 30 μM of doxorubicin, the viability and beating of spheres was significantly reduced, and reactive oxygen species production was increased. In addition, cell morphology was changed. Conclusion: The overall results obtained in the study showed that human embryonic stem cell-derived cardiomyocytes could be a useful model for in vitro evaluation of drug-induced toxicity.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/469
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