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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۷-۱۷۷
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عنوان انگلیسی Ps-146: Ultrastructure of Human Cumulus Oophorus: A Transmission Electron Microscopic Study on Vitrified Somatic Cells
چکیده انگلیسی مقاله Objective: Results show that cumulus cells of the adult have the genetic content to direct the development of newborn. However, techniques involving fusion of embryonic or fetal somatic cells with enucleated oocytes have become steadily more successful in generating cloned young. Cryobiology is the study of the effects of low temperatures on living organisms. In the future, it may be possible to cryopreserve human cells, whole human organs. The aims of this study are to quantify the ultrastructural changes that occur in the cumulus cells during vitrification. Materials and Methods: The study was divided into group A [(fresh cumulus cells (non-vitrified)] of human mature oocytes were used as controls) and B (cumulus cells of human mature oocytes were vitrified). Based on no significant differences in age, duration of infertility, infertile causes and transmission electron microscopy was used to compare the fine structure of vitrified cumulus cells of human mature oocyte and cumulus cells of human mature oocytes were non-vitrified. Results: The ultrastructural changes cumulus cell showed that the mitochondrial membrane is normall as well as the Crista of mitochondria and the number of lipid droplets and vacuole have been increased in vitrified cumulus cell, while in our control group which is not vitrified, the membrane of the mitochondria remain intact and no vacuole is observed. Conclusion: Vitrification did not damaged the organelles in vitrified cumulus cells Therefore, it is recommended to vitrify of cumulus cells for saving them permanently
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/471
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