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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۷۹-۱۷۹
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عنوان انگلیسی Ps-148: Evaluation of Adipose Mesenchymal Stem Cell Derived Neural Cells Exposed to Sera from Vitamin D Treated and Untreated Patients with Alzheimer’s Disease
چکیده انگلیسی مقاله Objective: Alzheimer’s disease (AD) has considered in part a reflection of the ordinariness of this progressive neurodegenerative disorder in recent years. Mature amyloid plaques in this disease are frequently associated with numerous dystrophic axons and dendritic processes that lie within or immediately around the fibrous amyloid deposit. Exist of large numbers of such senile or neuritic plaques in limbic and association cortices is probably the single most reliable neuropath logical marker of the diagnostic entity. Researches have revealed that vitamin D deficiency is associated with increased risk of neurodegeneration, while vitamin D consumption due to antioxidant properties can restrict neural damage. So we aimed to compare neural gene expression in adipose mesenchymal stem cell (ADSCs) derived neural cells which treated by sera from AD patients. Materials and Methods: We used sera from AD patients who treated and untreated by vitamin D for 3 months. First, HADSCs were isolated and differentiated to neuron like cells and then treated for two weeks with Sera from healthy individual (group 1), from AD patients who had not been treated by vitamin D (group 2) and from AD patients who treated by vitamin D for 3 months (group 3). Neurogenic differentiation of hADSCs evaluated by Quantitative real time polymerase chain reaction analysis (qRT-PCR). Results: Evaluation of the percentage of neural markers, Nestin, GFAP and MAP2 determined by RT-PCR. After treatment, induced cells were not significant difference for Nestin expression, in comparison with the control group, while the mean percentage of MAP2 and GFAP expression was significantly decreased in vitamin D untreated group relative to the control group and group which treated by vitamin D (p< 0.05). Conclusion: Overall, present results and its comparison with literature studies confirmed vitamin D not only possess neuromodulator properties also preserve neural cells from damage which induced during neurodegenerative disease like as AD and Multiple Sclerosis. Different in vitro studies have been tried to advance the mechanisms reduction of neurotoxicity, in addition to identifying new strategies that have higher therapeutic efficiency in neurodegeneration.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/473
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