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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۸۴-۱۸۴
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عنوان انگلیسی Ps-153: The Expression and Function of Toll-Like Receptor 2 in Proliferation and Osteogenic Differentiation of Human Bone Marrow-Derived MSCs
چکیده انگلیسی مقاله Objective: Adult stem cells with multipotent differentiation potential are present in almost all tissues of adult organisms. The main function of these stem cells is to support normal repair and rejuvenation of diseased and aging tissue. Multipotent mesenchymal stem cells (MSCs) seem to be a good candidate for cell therapy but information about factors controlling their proliferation and differentiation is not sufficient. Recently, studies have shown that chemokines and cytokines can affect MSC function. Toll-Like Receptors (TLRs), one of the main receptors in the innate immune system, can cause secretion of different cytokines like Interleukin 6 (IL6). Among TLRs, TLR2 has more different exogenous and endogenous ligands. In this study we investigated the expression of TLR2 during proliferation and osteogenic differentiation of human bone marrow MSCs (BMMSCs). Materials and Methods: MSCs were isolated from bone marrow of patients who were volunteers for cell therapy in Royan cell therapy center. The MSC identity of the isolated cells was confirmed based on the expression of surface epitopes including CD90, CD73, CD44, CD105, CD34, CD45, CD11b and CD31 and the differentiation capacity into bone, cartilage and adipose cell lineages. To examine TLR2 and IL6 expression pattern, we propagated MSCs in passage 3 (P#3) for three additional passages during which the expression of the TLR2 and IL6 were analyzed by qRT-PCR. Furthermore, bone differentiation culture conditions were established for P#3 MSCs and the expression pattern of TLR2 and IL6 was investigated at several time points including days 0, 7, 14 and 21. Results: The expression levels of TLR2 and IL6 were decreased as the number of cell passages increased. Since the proliferation capacity of MSCs decreased with passage, these results indicate that there would be a direct correlation between TLR2 expression and MSC proliferation capacity. This was in accordance with our findings on MSC osteogenic culture. According to our observation, at osteogenic culture, TLR2 and IL6 were upregulated by day 7 (proliferation phase of differentiation culture) followed by their downregulation towards the end of the differentiation period. This observation also emphasizes the reverse correlation between TLR2 expression and MSC bone differentiation. Conclusion: Taken together, our data indicates that there would be a direct correlation between TLR2 and IL6 expression and human MSC proliferation capacity and a reverse correlation between TLR2 expression and the MSC bone differentiation. These findings would be of importance for the field of stem cell therapy and regenerative medicine.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/478
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