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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۸۸-۱۸۸
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عنوان انگلیسی Ps-157: First Step of Regeneration Intervertebral Disc by Synthesis Biopoly mer Scaffolds and Identification Isolated Nucleus Pulposus Cells of Intervertebral Disc in Iran
چکیده انگلیسی مقاله Objective: Low back pain is a major economical and social problem nowadays. Inter-vertebral disc herniation and central degeneration of disc are two major reasons of low back pain that occur because of structural impairment of disc. Inter-vertebral disc contains three parts as follows: Annulus fibrosus, transitional region and nucleus polpusus which forms the central nucleus of the disc. Reduction of cell count and extracellular matrix, specially in nucleus polpusis causes disc degeneration. Different scaffolds (natural and synthetic) have been used for tissue repairing and regeneration of intervertebral disc in tissue engineering. Most scaffolds have biodegradable and biocompatible characteristics and also prepare a fine condition for proliferation and migration of cells. In this study, proliferation of NP cells of human intervertebral disc compromised in chitosangelatin scaffold with alginate scaffold was studied. Materials and Methods: NP cells derived from nucleus polpusus by collagenase enzymatic hydrolysis. They were derived from patients who undergoing open surgery for discectomy in Isfahan Alzahra hospital. Chitosan was blended with gelatin and glutaraldehyde was used for cross linking the two polymers. Then, alginate scaffold was prepared. Cellular suspension with 1× 105 transferred to each scaffold and cultured for 21 days. Cell viability and proliferation investigated by trypan blue and MTT assay. SEM was used to assert the porosity and to survey structure of scaffold.Eliza assay was used to assert production of extracellular matrix by NP cells. Results: MTT assay and demonstrated that cell viability and growth of third day had significant difference in contrast by first day in both scaffolds. Accordingly, there was a significant decreased in cellular viability from day 3 to 21. Results of cell count showed a significant increase of cell numbers for alginate scaffold but there was no similar result for chitosan-gelatin scaffold. Result of SEM showed porosity of chitosan-gelatin scaffold and attached NP cells into scaffold.Eliza assay showed significant increase in production of extracellular matrix from day 3 to 14. Conclusion: Alginate scaffold prepared a better condition for proliferation,viability and production of extra cellular matrix of NP cells in comparison with chitosangelatin scaffold. Results of this study suggest that alginate scaffold could be useful in in vivo studies and treatment.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/482
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