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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۸۹-۱۸۹
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عنوان انگلیسی Ps-158: Evaluation of Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cell on PLLA (poly-l-lactide acid) Scaffold Nonamembrane
چکیده انگلیسی مقاله Objective: Tissue engineering is combination of cells, engineering materials, and chemical and physical factors that its aim is improve or replace biological functions of tissue function in target tissues. scaffolds Is an important component of tissue engineering. These scaffolds are synthetic extracellular matrix used for cell attachment, proliferation and cell differentiation. In This project, the Nano membranes PLLA is used as scaffolds for proliferation and differentiation of bone cells. Materials and Methods: Nano-phase separation method was used to production of PLLA membrane with a suitable stability and morphology. So we first obtained a homogeneous polymer solution and then stretched on a flat bed. After that, mesenchymal stem cells have been removed from dental pulp and were cultured on PLLA nano-membranes to differentiate into bone. In the end of osteogenic period, for evaluation of morphology, proliferation and differentiation quantitative Alizarin red staining, MTT assay, and measuring alkaline phosphatase activity, SEM investigation was performed. Results: The results obtained in this project indicate that the nano-PLLA membrane expressed significantly greater alkaline phosphatase (ALP) activity (p< 0.001), indicating the early promotion of osteogenic differentiation. the level of differentiation was evaluated with the help of quantitative Alizarin red (P< 0.001) . MTT assay demonstrated that the MSCs grown on scaffold nanomembrane had significantly Higher(P< 0.001) cell viability than the tissue culture control .PLLA nano membrane due to nontoxicity and good biocompatibility significantly enhanced proliferation and Osteogenic differentiation, several times in mesenchymal stem cells. Conclusion: Nano PLLA membranes increases proliferation and differentiation of mesenchymal stem cell
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/483
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