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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۱-۱
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عنوان انگلیسی Is-2: OCT4B1, A Novel Spliced Variant of OCT4, Is Highly Expressed in Gastric Cancer and Acts As An Antiapoptotic Factor
چکیده انگلیسی مقاله Objective: The octamer-binding transcription factor 4 (OCT4) is involved in regulating pluripotency and self-renewal maintenance of embryonic stem cells. Recently, misexpression of OCT4 has been also reported in some adult stem as well as cancer cells; a finding which is still controversial. In addition to the previously described spliced variants of the gene (e.g., OCT4A and OCT4B), we have recently identified a novel variant of the gene, designated as OCT4-B1. Materials and Methods: In this study, we investigated a potential expression and function of OCT4B1 in a series of gastric cancer tissues and a gastric adenocarcinoma cell line, AGS. Using the Taqman real-time PCR approach. We have also analyzed the effects of OCT4B1 knock-down in AGS cell line treated with specific siRNA directed toward OCT4B1. Results: We have detected the expression of OCT4B1 in tumors with no or much lower expression in marginal samples of the same patients (p < 0.002).Our data revealed that interfering with the expression of OCT4B1 caused profound changes in the morphology and cell cycle distribution of the cells. Furthermore, down-regulation of OCT4B1 significantly elevated the relative activity of caspase-3/caspase-7 and the rate of apoptosis in the cells (more than 30%). Conclusion: All together, our findings suggest that OCT4B1 has a potential role in tumorigenesis of gastric cancer and candidates the variant as a new tumor marker with potential value in diagnosis and treatment of gastric cancer.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/767
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