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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳-۳
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عنوان انگلیسی Is-7: Real-Time Imaging of Tissue Formation Using Novel Noninvasive Method in Tissue Engineering
چکیده انگلیسی مقاله Conventional techniques for evaluating scaffold degradation demand large sample numbers and sacrificing animals at each time point, and this results in inaccurate conclusions due to their nonconsecutive single-timepoint information. Therefore, there is a desperate need for a facile and noninvasive analytical method to continuously monitor scaffold degradation and tissue formation. In this study, we have developed a functional biodegradable scaffold to investigate matrix degradation by employing invisible near-infrared (NIR) fluorescence. Briefly, an 800 nm NIR fluorescence emitting ZW800-1 was conjugated on the collagen scaffold that was made from small intestinal submucosa using conventional N-hydroxysuccinimide (NHS) ester chemistry. Degradation behaviors and tissue formation were monitored noninvasively in one animal over 4 weeks using fluorescence imaging and magnetic resonance imaging (MRI), respectively. To quantify the degradation pattern, conventional analytical methods such as histological and gravimetric assays were also carried out at the same time. In addition, we also developed an optimal condition for in vitro biodegradation studies, which can predict in vivo tissue formation along with scaffold degradation. This noninvasive method using NIR optical imaging and MRI will be a benefit to tissue engineers and save time, reduce the use of animals, and offer more accurate conclusions
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/772
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