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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۱۲-۱۲
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عنوان انگلیسی Os-3: Establishment of Pluripotent Embryonic Germ Cells by Inhibition of Signaling Pathwaysin A Chemically Defined Medium
چکیده انگلیسی مقاله Primordial germ cells (PGCs) are the unipotent cells with the ability to transform into a pluripotent state named as embryonic germ cells (EGCs) in vitro. EGC formation is more efficient, has a shorter culture period than somatic cell reprogramming and does not require exogenous genetic manipulation. Thus these cells are a suitable candidate to analyze mechanisms by which committed cells acquire a pluripotent state. In the present study we have attempted to elucidate a more defined, efficient protocol that derives EGCs by the suppression of TGF-β signaling and simultaneous inhibition of the ERK pathway. To achieve this, we applied two small molecules that inhibited the above mentioned pathways. Under this condition we noted that one day presence of stem cell factor (SCF) as a survival factor was adequate for PGC reprogramming, while the efficiency of this transformation was considerable.Moreover we have shown that this chemically supplied medium rules out the need for serum and feeder cell presence in culture. The resultant EGCs were characterized for pluripotency markers in gene and protein expression level and also for in vivo and in vitro differentiation capacity. Here we report that PGCs from different embryo ages can be reprogrammed into pluripotent stem cells through manipulating signaling pathways under chemically modified culture condition, regardless of animal genetic background.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/790
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