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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۱۷-۱۷
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عنوان انگلیسی Os-14: Generation and Maintenance of Human Embryonic Stem Cells-Derived Cadual Restricted Neural Precursors Cells by Small Molecules
چکیده انگلیسی مقاله Objective: Human embryonic stem cells (hESCs) have led to an important revolution in stem cell research and regenerative medicine. Recent attempts are designed to use of them in clinics and its combination with small molecules is another state-of-the-art features of biotechnology for increase our knowledge in stem cell biology. On the other hand, the availibilty of suitable cell sources for neurodegenerative diseases cell therapy seems negligible. Here, we try to achieve a proper, efficient and well characterize neural progenitor cell line by small molecules. Materials and Methods: Generating these hESCNPs was performed by using a combination of small molecules such as SB431542, CHIR99021 and Dorsomorphin after six days of induction. And aslo, we used common methods in cell biology such as Immuno fluorescence staining, flow cytometer and real-time PCR for characterize of these cell lines. Results: Our results show that the established hESCs-NPs line are highly expressed Nestin (above 80%),Pax6 and Sox1(more than 60%).These NPs were continuously propagated for approximately six months without losing their potential to generate astrocytes, oligodendrocytes, and functional neurons. Furthermore, spontaneously differentiation of these cell showed that they can be express general mature neurons proteins such as Tublin III(TUJ1),Neurofilament protein (NF),Glial Fibrilury Acidic Protein (GFAP). Finally, Real-time PCR analysis revealed that our established NPs gene expression profile is closely resemble to real and functional posterior neural progenitors cells. Conclusion: Here, we have shown that hESCs can generate large quantities of enriched stable proliferating NPs using of small molecules. Moreover, it was shown that expandable hESC-NPs were derived in high-density feeder-free hESC cultures without the addition of RA or noggin. Additionaly, In terms of the generation of expandable NPs, stability in passaging, and a constant neuro and gliogenic differentiation across many passages, our results are comparable with previously published reports on hESC-NPs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/801
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