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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۱-۲۱
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عنوان انگلیسی Ps-2: THY1 As A Reliable Marker for Enrichment of Undifferentiated Spermatogonia in The Goat
چکیده انگلیسی مقاله Objective: Spermatogonial stem cells (SSCs) are unique cells of testes that can restore fertility upon transplantation into recipient testes. However, use of suitable markers for enrichment of these cells is an important potential application. THY1 is an established conserved marker of SSCs in bovine, rodents and primates, but, there is no information available in goats. Materials and Methods: Following three rounds of enzymatic digestion of pre-pubertal goat testicular tissues, undifferentiated spermatogonia positive for THY1 were isolated by magnetic activated cell sorting (MACS) and were used for immunocytochemistry, real time PCR analysis of gene expression, protein expression, and transplantation into recipient mice. Results: Immunocytochemical analyses showed that significantly higher percentage of THY1+ cells were positive for PLZF and VASA as compared to unselected population. This result for PLZF was further confirmed at protein level. Real time PCR analysis revealed that expression of THY1, PLZF, VASA, BCL6B, UCHL1 as SCCs characteristic genes in THY1+ cells were significantly higher than in the initial population. Finally, transplantation of PKH26 labeled cells revealed that THY1+ cells had higher capacity for colony formation as compared to unselected cells. Conclusion: The results provide indications that THY1 surface marker can be reliably used for enrichment of undifferentiated spermatogonial in the goats.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/808
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