این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۳-۲۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-8: The Effect of Shear Stress on Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Endothelial Cells by A Perfusion Bioreactor
چکیده انگلیسی مقاله Objective: Vascular endothelial cells (ECs) are subject to hemodynamic stimuli such as fluid shear stress (SS) in vivo. SS is the most important mechanical factor which affects ECs; also SS induces differentiation mesenchymal stem cells (MSCs) into ECs. In the present study, the effects of SS on endothelial differentiation of human adipose-derived mesenchymal stem cells (hASCs) utilizing a bioreactor with different rates of SS is investigated by evaluation of vWF expression. Materials and Methods: The stem cells were studied in separate groups: control, chemical and mechanical groups. After isolation of hASCs from abdominal fat tissue of healthy women between 25-34 years old, characterization by flowcytometry and assessing multilineage differentiation potential, at passage 3 cells were used in experimental groups. In chemical test, MSCs treated by vascular endothelial growth factor (VEGF), for 7 days. In mechanical tests, a bioreactor was designed (in Research Center in Life Science Engineering, University of Tehran) to simulate blood flow and create SS with different amplitudes. For mechanical stimulation, MSCs were cultured on collagen type1 coated silicon tube and further exposed to SS in three different amplitudes (0.6- 1.06- 1.5 dyn/cm2) for 24 hours in bioreactor. hASCs without any specific treatment in normal culture conditions were considered as negative control and Human umbilical vein endothelial cells (HUVECs) were examined as positive control. For studying endothelial differentiation of hASCs, Real-Time PCR method was used to examine the expression of endothelial cell specific gene, von Willebrand factor (vWf). Results: Flowcytometry analysis shows expression of MSCs markers. Osteogenic and adipogenic differentiations confirm multipotency of hASCs. Results of Real- Time PCR indicate that by increasing SS, gene expression is also increased. However most of the gene expression is in chemical group. Conclusion: We proved that applying VEGF and increasingly SS amplitude, enhanced the expression of vWF. Thus, it is necessary to provide the microenvironments of native ECs in vivo during the in vitro tissue engineering process.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/814
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات