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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۴-۲۴
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عنوان انگلیسی Ps-11: The Effect of α-Tocopherol on The Osteogenic Differentiation and Morphologic Changes in Rat Bone Marrow Mesenchymal Stem Cells In Vitro
چکیده انگلیسی مقاله Objective: α-tocopherol as a strong antioxidant plays an important role in scavenging of free radicals. α-tocopherol has been found to have antioxidant and cytoprotective properties in cultured cells but its effect on osteogenic differentiation in bone marrow mesenchymal stem cells has not yet been determined. Materials and Methods: Rat bone marrow mesenchymal stem cells were extracted under strile conditions using flashing-out method. At the end of third passage, cells were divided to 2 groups control and α-tocopherol (15, 25 μM), for a period of 21 days in the osteogenic media (DMEM containing 10% fetal bovine serum, 10 mM β-glycerol phosphate, 10 nM dexamethasone and 50 g/ ml ascorbic 3-phosphate). The cell proliferation and viability, bone matrix mineralization , intercellular calcium deposition , extracellular calcium deposition , alkalin phosphatase activity and morphologic changes of the cells were measured using MTT assay, alizarin red staining , calcium kit , von kossa staining , alkalin phosphatase kit and Fluorescence staining respectively. Data was analyzed using way ANOVA and means difference was considered significanat at p< 0.05. Results: A significant rise in the cell viability, mineralization and calcium deposition , alkalin phosphatase activity,change of chromatin condensation, and cytoplasm morphology was seen in the cells treated with α-tocopherol. Conclusion: Since α-tocopherol caused a significant increase in viability, osteogenic differentiation in the rat bone marrow mesenchymal stem cells, therefore it can be used to treat osteoporosis.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/816
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