این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۴-۲۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-12: Effects of Amniotic Membrane Extract on Umbilical Cord Blood Mesenchymal Stem Cell Expansion
چکیده انگلیسی مقاله Objective: Umbilical cord blood is a good source of mesenchymal stem cell that can be expanded and stored in the cell bank and used in regenerative medicine. The objective of this study was to test whether amniotic membrane extract (AME), as a rich source of growth factors such as basic-fibroblast growth factor (bFGF), can promote the potential of cellular proliferation in UCB-MSC. Materials and Methods: UCBMSCs were cultured in the presence of bFGF and AME and compared with those cells cultured in media without supplementation. The proliferation rate of the growing cells was measured by BrdU assay. Duplication number and time were calculated. Cultured cells were assessed for the expression of stem cell’s specific markers by flowcytometry. The differentiation capacity of the UCBMSC toward osteogenic and adipogenic lineages was also evaluated. Results: BrdU assay showed a significant increase in the proliferation rate of AME -treated cultures. Similarly supplementation of the culture media with AME and bFGF led to a significant increase in the duplication number and a decrease in the duplication time without any change in the cell morphology. Both AME and bFGF alteredthe expressing of CD44 and CD105 in UCBMSC population. Adding bFGF but not AME to the culture media favored the differentiation potential of UCBMSC toward osteogenic lineage. Conclusion: AME may enhance the proliferation rate and duplication number of the stem cell through changing the duplication time.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/817
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات