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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۴-۲۴
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عنوان انگلیسی Ps-13: Histopathological Evaluation of Adipose-Derived Mesenchymal Stem Cell on Cartilage Defects of Knee in Male Rabbits
چکیده انگلیسی مقاله Objective: Partial-thickness defects evolving in mature articular cartilage do not heal spontaneously. Tissue engineering has long been investigated to repair articular cartilage defects. The current study was designed to observe chondrogenic differentiation of adipose derived stem cells (ASCs) to repair partial-thickness articular cartilage defects in non-weight bearing area in adult rabbit. Materials and Methods: A partial-thickness (without penetration of the subchondral bone, with 4 mm in diameter) cartilage defect was created in the medial femoral condyle of twelve adult Dutch rabbits. Autologous mesenchymal stem cells isolated from subcutaneous adipose tissue of the same rabbit harvested were injected intra-articularly after the creation of the defect. Defects without treatment were used as controls. The rabbits were sacrificed at 4 and 8 weeks for histological analysis. Healing of the defect was investigated histologically using haematoxylin and eosin, safranin-O staining and toluidine blue. Results: The results showed that in treatment groups, at 8 weeks post-surgery, the defects were resurfaced with hyaline-like tissue and an ideal interface between the engineered cartilage, the adjacent normal cartilage, and the underlying bone was observed. In contrast, at 4 weeks post-implantation was partially filled with repair tissue, but only half of the repair tissue was hyaline cartilage. Defects were only filled with fibrotic tissue in controls. The findings showed significant differences in the quality of cartilage between ADSCs-injected groups compared to control group, particularly at 8 weeks after surgery Conclusion: Stem cells that exist in the adipose tissues are able to renew themselves through cell division without changing their phenotype and are able to differentiate into chondrogenic lineage under certain physiological or experimental conditions.These findings suggest that it is feasible to repair articular cartilage defects andmaintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy with implants generated by seeding autologous ADSCs, without in vitro differentiation for cartilage tissue engineering.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/818
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