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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۶-۲۶
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عنوان انگلیسی Ps-16: Propagation of Caprine Spermatogonial Colonies by Different Growth Factors
چکیده انگلیسی مقاله Objective: Spermatogenesis depends on spermatogonial stem cell self renewal and differentiation, but these cells are very few in each testis approximately 0.02-0.03% in mouse testis. In the present study, cell lines from neonate caprine testis were cultured and characterized and the effect of growth factors were investigated. Monitoring cultured cells after 4 and 7 days revealed colonies resembling spermatogonial colonies contained spermatogonial stem cells (SSCs). Materials and Methods: Growth of these colonies depended on the growth factors: leukemia inhibitory factor (LIF), epidermal growth factor (EGF), glial-cell derived neurotrophic factor (GDNF), GDNF receptor α-1 (GFR- α-1), Insulin like growth factor-I (IGF-I) and basal fibroblast growth factor (bFGF). Basal media (BM), BM plus GDNF and BM with GDNF and GFR- α-1 had the most number of colonies in compare with BM contains GDNF, IGF-I and bFGF, BM with GDNF, EGF and LIF and BM plus all growth factors in day 4. Results: However seven days after culture, the least and insignificant decrement in number of colonies observed in media supplemented with IGF-I and bFGF. In day 4 the least number of colonies were achieved in effect of GDNF, EGF and LIF. In the other hand, these growth factors had putative effects on proliferation of somatic cells. The basal media was supplemented with all growth factors provided good conditions for both somatic cells and spermatogonial colonies. Furthermore we determined spermatogonial colonies by immunocytochemistry with SSC, germ cell and somatic cell markers. Conclusion: Characterization of these colonies revealed that all aforementioned markers were positive in all colonies of experimental media. RT-PCR analysis of SSC markers and transplantation of colony cells confirmed that media containing GDNF+IGF-I+bFGF was the best mixture for caprine SSC maintenance.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/821
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