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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۷-۲۷
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عنوان انگلیسی Ps-17: Neural Differentiation of Adipose Tissue-Stem Cells Is Improved Following Coculture with Embryonic Stem Cells
چکیده انگلیسی مقاله Objective: The aim of the present study was to evaluate neural differentiation of adipose tissue-derived stem cells (ADSCs) following coculture with embryonic stem (ES) cells. Materials and Methods: ADSCs were isolated from the inguinal adipose tissue of mice. Third-passaged ADSCs were cultured to reach 90% confluency. Neural differentiation of ADSCs was performed in DMEM supplemented with Knockout Serum Replacement (KoSR). RoyanB1 ES cells were seeded into Transwell-Clear inserts of 6-well tissue culture plates and cocultured with third-passaged ADSCs for two days. Culture inserts were then removed and neural differentiation was initiated in DMEM supplemented with KoSR. Two weeks after the initiation of differentiation, the expression of neural-specific markers in the differentiated ADSCs was assessed by RT-PCR, quantitative real-time PCR and immunocytochemistry. Results: 24 hours after the initiation of differentiation, ADSCs showed a neuron-like morphology. Coculture of ADSCs and ES cells improved neural differentiation of the ADSCs. Differentiated ADSCs showed the expression of PAX-6, Nestin, NSE,NeuN, NEFL, TH and ChAT mRNAs. Neuron-specific proteins like MAP2, NEFL and β-tubulin III were also detected in the differentiated ADSCs by immunocytochemistry.Based on quantitative real-time PCR, the expression of neural specific genes increased after Coculture of ADSCs with ES cells. Conclusion: ADSCs exhibited an excellent potential for differentiation toward neurons, and neural differentiation of the ADSCs improved after coculture with ES cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/822
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