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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۹-۲۹
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عنوان انگلیسی Ps-21: miR-22 As A Stem Cell Specific MicroRNA Reveals Differential Expression in Tumoral and Non-Tumoral Breast Tissue
چکیده انگلیسی مقاله Objective: MicroRNAs (miRNAs) are a major class of small endogenous RNA molecules that post-transcriptionally inhibit gene expression. Many miRNAs have been implicated in several human cancers, including breast cancer. Breast cancer is the most frequent form of cancer in women and Cancer stem cells (CSCs), or cancer cells with stem cell properties, have been reported in many human tumors and are thought to be responsible for tumor initiation, therapy resistance, progression, relapse, and metastasis. The cancer stem cell hypothesis postulates that tumors are maintained by a self-renewing CSC population. MicroRNAs play critical roles in normal stem cell functions during development, have emerged as important regulators of CSCs as well. Several micro- RNAs, including miR-22, are highly expressed in mammary progenitor cells, while others, including let-7, are depleted so miR-22 can act as a self-renewal microRNA in breast cancer stem cell (BCSC) which is located in l7p13.3 genomic locus. The aim of this study is to evaluate the expression alteration of miR-22 as a BCSC specific marker. Materials and Methods: A matched case-control study was conducted that included tumor and matched nontumor surgical specimens from patients diagnosed with breast invasive dactal carcinoma. formalin-fixed, paraffin- embedded (FFPE) samples was prepared for RNA extraction with using xylene-ethanol method then Total RNA was isolated using TRIzol reagent. Specific stemloop primer was designed and cDNA synthesized particularly. The expression of MicroRNA was evaluated by Real-time PCR. Results: According to our data, miR-22 expression revealed alternation in tumor versus non-tumor samples of breast tissue. Also indicated our stem-loop primer synthesized cDNA specifically. Conclusion: Despite the alternation expression of miR- 22 in breast tumor could claim the presence of CSCs in tumor cell population. However, introducing miR-22 as a discrimination factor of tumor state is under study and needs further investigation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/826
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